Re: Avidin/APC conjugation

From: Mario Roederer (Roederer@Beadle.Stanford.EDU)
Date: Tue Jun 24 1997 - 15:48:00 EST

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Hi Adrian.  You can make Av-APC using essentially the protocol that I put on the
web for making Annexin-APC.  Find the protocol at
"http://cmgm.stanford.edu/~roederer/abcon".  Just modify the molar ratios to
take into account the difference in molecular weight between avidin and annexin.
I have not optimized this protocol for avidin; you may want to try a couple of
ratios of APC to avidin.

mr

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