INDO-1 Calibration

From: HANDLEY@sorter.dfci.harvard.edu
Date: Mon Jun 16 1997 - 09:27:13 EST


Hello Everyone!

Does anyone know if there is a kit available to help calibrate a flow cytometer
for calcium flux using Indo-1?  I have a researcher who wants to be able to
compare values between experiements, and I have suggested using ionophore
and EGTA to help with this, but he seems reluctant to do all the calculations
involved.  Any suggestions?

Thanks,
Maris

Maris Handley
Dana-Farber Cancer Institute
Boston, Ma 02115
(617)632-3179

handley@sorter.dfci.harvard.edu



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