Hugh,
I think FMC7 is very useful. It is also positive on prolymphocytic
leukemia. It even helps in those difficult cases that are most consistent
with CLL or Mantle cell. For example- what about cells with nuclei that are
not very round, CD5+, CD19+, CD23 dim and FMC7 bright?
Maryalice
>MaryAlice,
>
> Should you run FMC7 if you have cd19+, cd23-/cd5+ lymphoma? Our
>pathologist, when I approached him with this, believes it isn't
>necessary. You already, if the histology agrees, have a mantle zone
>presentation without FMC7 positivity.
>
> For our lymphoma panels we run cd3/cd19, cd5, cd10, cd23, kappa, lambda
>for B, appropriate T markers if not B.
>
>thanks,
>
>hugh johnson
>flow lab guy
>st vincent infirmary medical center
>little rock, ar
>>Maryalice Stetler-Stevenson wrote:
>>
>> I can't cite statistics but from our clinical experience, FMC7 is extremely
>> sensitive for mantle cell but not specific (many other lymphomas are FMC7
>> positive). I have never had a real mantle cell lymphoma that was FMC7
>> negative (most consistent with Mantle cell doesn't count as these are
>> usually something in between real mantle cell and something else). It is
>> useful in differentiating CD5+ neoplasms with the appropriate morphology. A
>> CD5+ neplasm that is FMC7+ and CD23- and has small lymphoid cells with
>> compact chromatin as well as some what cleaved nuclei is mantle cell. But
>> then of course, nothing stands alone in diagnosis of neoplasia. If you have
>> a specific example, what are the other antigens studied?
>>
>> Maryalice
>>
>> >HEY,
>> >DOES ANYONE KNOW THE SENSITIVITY AND SPECIFICITY OF FMC7 IN THE FACE OF A
>> >PICTURE THAT APPEARS TO REPRESENT INTERMEDIATE CELL (MANTLE CELL OR ZONE)
>> >LYMPHOMA IN HUMANS (FOR YOU PURISTS). THANKS SO MUCH, MELISSA L. BEAL, M.D.
Maryalice Stetler-Stevenson
Director Flow Cytometry Unit
Laboratory of Pathology, NCI, NIH
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