--- On Mon, 9 Jun 1997 12:07:15 +0200 Michel Canton <mcanton@wcube.fr> wrote: >In the course of some R&D work on apoptosis, we "accidently" came out with a >good method to evaluate the Fas receptor occupancy in a highly quantitative >and very standardized manner. >What would be the application(s) of such an approach? The ability to define those Fas expressing cells already triggered by Fas ligand is potentially very important in the context of HIV infection as well as certain autoimmune disorders. Although other regulatory molecules are definitely involved [bcl-2 family, etc], the Fas/Fas L interaction may be a requisite step for peripheral T cell deletion. For example, PBMC from HIV infected and uninfected individual s both express Fas, but only the cells from infected people undergo rapid in vitro apoptosis-presumably triggered in vivo. So an application might be to quantitate the % of cells with Fas R already occupied. Regards, Tom -------------------------------------------------------- Thomas W. Mc Closkey, Ph. D. Director, Flow Cytometry North Shore University Hospital Biomedical Research Center 350 Community Drive Manhasset, Long Island, New York 11030 ph: 516-562-4844 [office]; 516-562-1135/4641 [lab] 6/10/97 1:01:25 PM E-mail: thomasm@nshs.edu --------------------------------------------------------
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