Green HeNe Laser - TexasRed/Cy3

From: O. Joseph Trask, Jr. (cytometry.osu.edu@postbox.acs.ohio-state.edu)
Date: Tue Jun 10 1997 - 09:36:22 EST


Greetings -
Is anyone out there using a Green NeHe Laser (~543nm) to excite Texas Red or
Cy3 for any  type of immunophenotyping analysis, if so, how much output
power?  Melles Griot has a 2mW Green NeHe Laser (543nm), but is 2mW output
power enough for immunofluorescence?   I know 50mW from the 568nm line on a
Krypton Laser works well.  All comments will be appreciated.
Regards,
O. Joseph Trask, Jr.
The Ohio State University 
Comprehensive Cancer Center
Analytical Cytometry Laboratory 
Tel: 614/292-FLOW(3569)
Fax: 614/292-7335
E-Mail: cytometry@osu.edu



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