Greetings - Is anyone out there using a Green NeHe Laser (~543nm) to excite Texas Red or Cy3 for any type of immunophenotyping analysis, if so, how much output power? Melles Griot has a 2mW Green NeHe Laser (543nm), but is 2mW output power enough for immunofluorescence? I know 50mW from the 568nm line on a Krypton Laser works well. All comments will be appreciated. Regards, O. Joseph Trask, Jr. The Ohio State University Comprehensive Cancer Center Analytical Cytometry Laboratory Tel: 614/292-FLOW(3569) Fax: 614/292-7335 E-Mail: cytometry@osu.edu
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