Hello All! I am back again with a question regarding multi-user instruments (ours is a FACSCalibur). Our institute does not believe in paying one person to monitor the instrument (yet), so our current setup involves a monthly rotation where each lab is responsible for the instrument. In that month, that lab is required to run beads and do the monthly instrument cleaning protocol. The only other cleaning that is done is by the user. Each user is required to clean with 10-20% bleach for 10 min followed by deionized water for 10 min. Guess what? This hasn't been working. I have had to do the monthly maintenence cleaning three times over the past two weeks. I suspect that some have not been doing their post sample run cleaning, but of cours,e I have no way to prove that this is the case (do I?). To all people in similar situations: HELP - I am running out of ideas. The FACSCalibur was purchased specifically because of its hands - off reputation. Any suggestions on how to improve this nasty situation? I am sick of having to clean the instrument for 45 minutes every time I want to run samples. Thanks! Abby -- ********************************************************************** Abby Allen Center for Blood Research 800 Huntington Ave. Boston, MA 02115 allen@cbr.med.harvard.edu **********************************************************************
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