Hello, Flowers: Last week, Amy Crosbie asked about anyone with experience with canine immunophenotyping. I understand that she is more interested in leukocytes. I shall respond to the Mailing list because we have worked mostly on platelets and someone could also be interested in canine platelets. Earlier this year we have been involved with some work for some pharmaceutical companies to establish the quantitative canine, pig, macaca cynomolgus platelet Glycoproteins immunophenotypes under various conditions. To achieve this task we have used the QIFI technology which we developed many years ago. This technology, derived from our CYTOQUANT Gp kit and adapted to these specific needs, allowed us to complete these projects very rapidly with great success by testing platelets directly in citrated whole blood with no washing step. The results were obtained after validating various mouse anti human Gp monoclonal antibodies. Unfortunately we did not have the time to go further and to establish the quantitative immunophenotypes on granulocytes for these various animal species, commonly used in the pharmaceutical R&D. The overall great advantage of this method is that one does not need to FITC label or PE label the monoclonal antibodies. Therefore the screening of the best suitable reagents is easy. Another big advantage is that the method is very standardized and works on any type of flow cytometer. Hoping these informations may be of some help to Amy and to other people, Michel Canton, PharmD Managing Director BioCytex 140, Chemin de l'Armée d'Afrique 13010 Marseille France Tel: +33 (0)4 91 94 29 39 Fax: +33 (0)4 91 47 24 71 E-mail: mcanton@wcube.fr
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