It should be possible to remove the oligos on the outside of the cell with a brief treatment with protease free DNAse. We have used a protocol like this to examine the uptake of oligos by imaging. A second method could include "pulse" labeling once you are sure the oligos are getting into the cells.
This archive was generated by hypermail 2b29 : Wed Apr 03 2002 - 11:49:49 EST