I have a query regarding detection of fluoresceinated oligos intracellularly by FACS. Is it possible to distinguish between externally bound liposome/oligo complexes and internalised intracellular complexes by FACS following transfection of mammalian cells? I know that I could use a microscope (heaven forbid!) but the number of samples and the need for quantitation w.r.t. transfection efficiency preclude that option (plus I'm very attached to my FACSort so would always choose that over a microscope if I had a choice). Maybe there is a way to strip off non-internalised oligos so you can be sure you're measuring only internal fluorescence? Any tips from users who have tried this would be very much appreciated. Thanks in advance, Michelle Miller Johnson and Johnson Research GPO Box 3331, Sydney NSW 2001 Australia Ph 61 02 9360 9377 Fax 61 02 9360 9813 Email jjph13mm@angis.su.oz.au
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