Re: Jurkat surface-marker

From: Kevin G Waddick (waddi002@maroon.tc.umn.edu)
Date: Wed Jun 04 1997 - 10:36:45 EST


 Jurkat cells express CD2, which is a co-stimulatory adhesion receptor on 
T-cells that has CD58 as its ligand.  I am sure that you can get a FITC 
conjugate of CD2 from virtually any source of MoAb;  we got ours from 
Coulter/Immunotech.

Kevin G. Waddick, Ph.D.
Staff Scientist
Hughes Institute
2685 Patton Road
Roseville, Minnesota 55113
(612) 628-0598
(612) 628-9891  Fax

On Tue, 3 Jun 1997 kjartan.egeberg@medisin.ntnu.no wrote:

> 
> Dear flowers.
> 
> In a flowcytometric study of adhetion of Jurkat cells to target cells, I
> need a good surface-marker that is direct conjugated to FITC. Jurkat cells
> do stain for CD3-FITC, but weak. They are negative for CD4, CD8, CD7 and
> CD45. 
> A tip of a good marker will be highly appreciated !
> 
> Thanks in advance and have a good flowday.
> 
> Kjartan Egeberg
> Inst. of Canser Research and Mol. Biol.
> NTNU 
> University in Trondheim
> E.mail: kjartan.egeberg@medisin.ntnu.no
> 



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