Reporter gene + cytoplasmic Ab

From: Mark A. Miller (mamiller@biochem.dental.upenn.edu)
Date: Mon Jun 02 1997 - 14:00:08 EST 

Does anyone have any experience with a reporter gene, such as LacZ + FDG 
or GFP in conjunction with cytoplasmic immunostaining?  My colleague 
would like to track a transient transfection in fibroblasts and measure 
elastin levels with a fluorescent MoAb at the same time.

My question is this:  will permeablization (to get the MoAb in) allow 
the GFP or beta galactosidase/FDG leak out?  What about maintaining 
the structure/function of the reporter system?  Is there a way to do 
both kinds of staining at the same time?

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