Hi Flowers, My question concerns pH measurment using Snarf and the ratio setup. I know that with calcium flux (using indo-1) that your settings have to be on linear (gain of 1) for fl4 and fl5(using the facs Vantage).Is this also the case for pH ratio measurements which also use a ratio for fl2 and fl3. Could I play around with the gains instead of leaving them at gain 1 for fl2 and fl3. Thank you in advance...Ken McDonald
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