Lighten up flowers. If one cannot ask a question of the list without some self appointed flow expert jumping on the questioner for stupidity or even worse unethical procedures, it would seem that the list will only be of use and subscribed to by an elite few. I also find that a lot of the posting are of no interest to me at the present time but as people working in a scientific or clinical environment should we not be quite so narrow in our outlook. I find it interesting that frequently the answers to quite mundane questions are provided by Dr Shapiro or Dr Darzynkiewicz two people who have forgotte ( maybe) more flow than the rest of us will ever know. If they have the time to be patient with those of us who are not so expert then I think that no one else is too busy to read the first two or three lines and see if the posting is of interest. I am sorry that more people do not reply to everyone but only to the person asking the question, because quite often I have thought that the information might be of use at a future date. I know there will be people annoyed because I am keeping this going, but as they started the name calling they must expect that they will have the muck thrown back. Jill Jill Martin, Manager, Molecular Biology & Flow Cytometry Core Johnson Research Building Mayo Clinic Scottsdale 602-301-6071 email: jmartin@mayo.edu
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