Hi Lyndal, I am responding via the list because your e-mail system bounced back my letter. I'm glad that the gentle ethanol fixation protocol described by us a few years ago worked; we've used it for a number of applications to "walk the line" between the need for good cell permeablization for cell cycle analysis and preservation of surface markers for immunophenotyping. Let me know if you need any additional assistance/advise. Bill
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