Cell membrane protein

From: Andy Riddell (ar3@mrc-lmb.cam.ac.uk)
Date: Thu May 22 1997 - 11:43:41 EST

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An investigator requested the following info.


I want to show by FACS that a protein is cell
surface expressed.  In western blotting reduction
is required for detction of this epitope tag,
indicating Ab inaccessiblity in 3D structure.  Are
there any methods to partially disrupt cell
surface proteins, and hence detect this epitope,
while maintaining cell viablility for FACS ?


Any info would be greatly appreciated.


Thanks in advance.
	Andy Riddell

 PNAC Division
 MRC Laboratory of Molecular Biology,
 Hills Road
 Cambridge   CB2 2QH
 U.K.

 tel: (01223) 402 218
 fax: (01223) 412 178

 e-mail: ar3@mrc-lmb.cam.ac.uk

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