>What wavelength(s) excite rhodamine? > >From the reference to -dull and -bright cells, I assume you're interested in rhodamine 123, which is best excited at 488 nm (its absorption maximum is at 485 nm). There are a lot of rhodamine dyes, and they usually are identified with supplementary letters (e.g., rhodamine B, absorption about 550 nm) or numbers (e.g., rhodamine 101, the chromophore of Texas red, absorption about 590 nm) or with the relevant chemical substituents added to the name (e.g., tetramethylrhodamine, usually conjugated as TRITC, absorption about 550 nm). None of the three rhodamine dyes just named excite enough at 488 to be useful; rhodamine 123 is about the only commonly used rhodamine dye with an absorption wavelength in that neighborhood. -Howard
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