Dear Adrian: We agree with Pete Macardle that rib specimens from cardiac surgery (and sometimes from kidney DONOR transplant) are very good for normal bone marrow studies. Also, it is possible to work with bone marrow derived from head femur obtained during ortopedic surgery (hip replacement). The latest one is more complicated and time consuming than flushing media through the rib but at the end we have to work with what is available in house. Anyhow, rib is better. Also, you can freeze the remaining of the sample in case that you want to analize another antigens. Although later studies are more complicated because of apoptotic cells (neutrophils and monocytes). It is important to have i) a good blocking system to avoid unspecific antibody binding, ii) separation of apoptotic cells based on FSC and SSC, iii) plus the antibody coktail suggested by Brad Sherburne (that looks fine). Lymphocytes and blast usually remains unchanged. We hope that this comments can help you to find a way to analyze this difficult issue. Please, do not forget. Good luck is also very important! Cesar Nunez, MD. University of Manchester School of Biological Sciences G.38 Stopford Building Oxford Road Manchester M13 9PT
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