Dear Adrian,
I want to echo Brad Sherburne's comments. Your questions are valid
and not easy ones. When I started analyzing bone marrows for leukemia and
low level lymphoma I had the benefit of learning (at flow courses) from two
great clinical flow cytometrists- Raul Braylan and Mike Borowitz. They
taught me me the basics of how to chose a gate in analysis of abnormal
hematopoietic populations. For Bone marrow, we always use CD45 as a third
color. This allows us to get a pure lymphoid gate for lymphomas and this
increases our sensitivity when looking for low level involvement. We also
like the way SSC vrs CD45 shows mature cells separated from blasts- very
useful for leukemias. The Clinical Cytometry Society is sponsoring a course
in Charleston this August. Raul will be teaching analysis of
lymphoproloferative disorders and Mike will cover leukemias. In addition,
Bob Bray and Larry Lamb will bring everyone up to speed on bone marrow
transplant flow, Ken Bauer will cover cell cycle analysis and Vince Shankey
will introduce all to molecular markers of neoplasia. Ric Duque will give a
lecture on how one approaches flow data. The Flow Cytometric
Immunophenotyping of Leukemias and Lymphomas Consensus guidelines will also
be covered. If you want information about the course send me your address.
The CCS meeting immediately follows the course. I have always found the CCS
meeting to be a great place to learn something new about clinical flow and
to ask experts questions about problems in the lab. The atmosphere is very
friendly and open to questions. I highly recommend the CCS meeting to any
one in clinical flow. The contacts you make there will help your throughout
the year. I also highly recommend the course.
Maryalice
Maryalice Stetler-Stevenson
Director Flow Cytometry Unit
Laboratory of Pathology, NCI, NIH
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