>there is a lot of data published and unpublished supporting the lack of >correlation between the level of CD69 expression and the level of >proliferation, one event is measured at 4 hrs or so the other after 3 or 4 >days, obviously a lot can happen in between, differentiation, apoptosis, >etc. In my experience it is clear that an increase in CD69 expression >does not necessarily indicate that a cell is going to divide. The vise >versa, I have not experienced nor heard of ie. induced proliferation >without an increase in CD69 expression. This is a novel observation and >warrants a >repeat. If it is indeed reproducible, I have at least one question. In >your assay system are the cells tested for CD69 induction stimulated in >whole blood whereas the cells tested for proliferation stimulated as >isolated PBL? Clearly I am thinking that you have removed some inhibitory >substance through the isolation of the PBL. Just a thought. > >Maurice R.G. (Mo) O'Gorman >Director Diagnostic Immunologyand Flow Cytometry Laboratories >Children's Memorial Hospital >Assis. Professor Pediatrics >Northwestern University Medical School
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