Dear FLOWers I have recently started to look at oxidative burst as a means of assesing Phagocytosis. My problem is that when I add the OxyBURST-Green reagent at the concentrations suggested by molecular probes the cell population moves dramatically to the origin on Forward and Side scatter. This would indicate to me that the cells ( this is a tissue culture line) are quite unhappy. Under the scope there seem to be very few intact cells. (and unsuprisingly alot of debrie). Does anyone out there (not on the Heavens Gate tour) have any succesful experiences with these reagents, and would you be willing to communicate with me? Thanks For your Time Pb
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