Has anyone noticed staining of cytokeratin on the cell _surface_ of epithelial cells? These ovarian tumor cells exclude PI, and have 'normal' forward vs side scatter. Other PI+ cells in the mixture stain for cytokeratin, as do some other PI negative cells that clearly have reduced forward scatter. I assume both these two latter populations are nonviable or nearly so, and might be nonspecifically staining with the anticytokeratin reagent. While i understand PI exclusion is not foolproof for 'cell death', the absence of PI staining and appropriate light scatter make me wonder about the possibility of real cytokeratin expression on the cell surface of a minority of these cells. My idea at this time is to sort out the cells and see if they grow and maintain this surface expression. Any other suggestions, experience or ideas? thankyou ```````````````````````````````````````````` Dr. Frederic I. Preffer preffer@helix.mgh.harvard.edu Department of Pathology- Warren 525A 100 Blossom St Massachusetts General Hospital Boston MA 02114 v(617) 726-7481 fax(617) 724-3164
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