Dear FLOWers, I have an ACAS 570 and am having problems trying to release Ca++ from internal stores, without having any Ca++ enter from external solution in cultured endothelial cells. Caffeine does not do so. Neither does ryanodine. Thapsigargin gives equivocal results and we need another agent. Does anyone have any suggestions for another agent? Thanks in advance, Steve Gaffin U.S. Army Research Institute of Environmental Medicine Natick MA 01760-5007 Tel. 508-233-4867
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