TO: <Michael_Ormerod@compuserve.com> Dear Dr.Ormerod, Thank you *very much* indeed for your proposition regarding your book on CD-ROM! We are very interested in receiving a copy. I must say that the flow cytometry is still an exotic in Ukraine. There are only 4 Facscans in Kiev and another one in Lviv. As I know these machines are used only for routing work: 2-color phenotyping (with no attempts to measure MFI) and sometimes cell cycle. As I know *some* people here is doing incorrectly even this humble flow job because of lack of some BASIC knowledge about cytometry. For example, until recently my colleagues and I were deadly sure that switching the DDM on would eliminate all doublets DURING acquisition (why not? the machine looks so smart..). As a result we have a lot of files with an FL1-A parameter written (a default parameter when the DDM is on). I got a lot of fun when I found an article in Cytometry explaining what the FL2-A/FL2-W gates are for. Maybe, it's time to open "cytometry.alt.humor"? There was even funnier story with compensation (many thanks to Dr.Roederer for his excellent Tutorial!). There are already several issues of Cytometry in one library in Kiev (thanks to the initiative of Prof. Darzinkewitch et al.). It is a very good journal but it cannot replace something like a handbook. So, I want to thank you again for your proposition. Sincerely yours, Vadim Chromyak, <gerund@olinet.isf.kiev.ua> Kiev, Ukraine. PS. Unfortunately, I will be out of town until 05/12/97. So, excuse me if there will be some delay with answer. PS#2. An exemple of black humor: - Daddy, please, I don't want to see granny! - Shut up and dig! (sorry)
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