Dear flow people does anyone have experience with a flow-based proliferation method that doesn't require a lot of cells? i'm looking to measure cytokine dose response from cell lines. the Brdu kits call for about a million cells per test. ideally, i want to titer the cytokine and culture the cells in 96 well microtiter plates, and seed with 10E+03 or 10E+04 cells per well. greatly appreciate if anyone has a method that can measure cell prolif with so few cells or an alternative to 96well plates that can give standard curve information. woo
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