On 14 Apr 97 at 9:28, Peter Lopez wrote: > > Hello, > > I have been doing some E.Coli analysis and sorting and was > wondering if anyone had a favorite sheath fluid to use for these > bugs. I am looking to make them as happy as possible during sort > cloning. Thanks for any tips. Many of the commercial preps are unsuitable for bacteria because of the high sodium content - they are designed to keep animal (blood) cells happy. I haven't done sorting with coli but Micrococcus luteus seem ok (i.e. grow afterwards) when sorted in a sheath fluid containing 150mM KCl and 10mM Hepes (pH 6.8 with KOH). This was with cells that weren't exactly happy to begin with : Kaprelyants AS, Mukamolova GV, Davey HM, and Kell DB: Quantitative analysis of the physiological heterogeneity within starved cultures of Micrococcus luteus using flow cytometry and cell sorting. Appl. Env. Microbiol. 62:1311-1316, 1996. Haze ---------------------------------------------------------- | Hazel Marie Davey hlr@aber.ac.uk | |Sefydliad y Gwyddorau Biolegol*Inst. Biological Sciences| |Prifysgol Cymru * University of Wales| | ABERYSTWYTH, Ceredigion, CYMRU / WALES SY23 3DA | | http://pcfcij.dbs.aber.ac.uk/index.htm |
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