Leslie- The Rainbow Calibration Particles from Spherotech (available through Pharmingen, 1-800-848-6227) have worked well for us for years. They have a dye mix in them which excites at UV, 488nm, and dye laser emmission. Using standard Texas Red and APC collection optics we regularly measusre cv's of less than 7% with 598 nm dye laser excitation. The intensity in these channels is approximately two log decades above the background for unstained lymphoid cells. With 488nm excitation signal intensities are similar to the dye laser measurements with better cv's. -Marty Bigos Stanford Shared FACS Facility
This archive was generated by hypermail 2b29 : Wed Apr 03 2002 - 11:49:38 EST