Sticking it to PBMCs

From: sgaffin@NATICK-CCMAIL.ARMY.MIL
Date: Wed Apr 09 1997 - 17:22:15 EST 

     
     To All Fluorescence Imaging People:
        I have an ACAS 570 and want to study peripheral blood mononuclear 
     cells in a thermoregulated cell superperfusion chamber.  My problem is 
     that these cells won't stick to the glass coverslip at all unless I 
     use CellTak, but even when I use Cell-Tak, they float away when there 
     is any movement of the superperfusing fluid.  As a result, I can't 
     change the medium surrounding the cells.  I have tried the usual 
     adhesives like PEG, gelatin, and polylysine
        Does anyone know of a "superglue" for PBMCs?
       
     Thanks for considering this.  
                      Steve Gaffin
                      US Army Research Institute for Environmental Medicine
                      Natick MA 01760-5007
                        Tel 508-233-4867

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