At 01:21 PM 4/7/97 CST, you wrote: > >Does anyone have a good yeast cell cycle method? In particular, we need to >eliminate a problem with the yeast clumping and try to get good single cell >suspension. Have been using PI, but will try any method/dye that works well. >Thank you, Martha >Martha Kirby >NIH, NHLBI, HB >Bldg. 10, Rm 7C212 >Bethesda, MD 20892-1652 >kirbym@helix.nih.gov >301-402-0277 > > Check out: Methods in Cell Biology : Flow Cytometry ,Second Edition Part B , Chapter 27p 458. Several of our investigators have used this with success. Larry Arnold Director, Flow Cytometry Facility Univ. of North Carolina at Chapel Hill
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