I know that Immunotech purified freeze-dried clones do not contain azide. They may have an antibody that can be used in your blocking study. They are now marketed by Coulter. Try their technical assisatance line at 1-800-526-7694. Hope it helps! Joe ---------- > From: Calman Prussin <CPRUSSIN@atlas.niaid.nih.gov> > To: Cytometry Mailing List <cytometry@flowcyt.cyto.purdue.edu> > Subject: anti-HLA mAb > Date: Wednesday, April 02, 1997 8:24 PM > > > I am looking for an antibody against human MHC II for use in tissue > culture to block antigen presentation. Ideally it would recognize a > monomorphic determinant on DP, DQ and DR and be sold without azide. > Any ideas? I have yet to have much luck after calling a half dozen > companies and another half dozen friends. Thanks, > > Calman > > _______________________ > Calman Prussin > Laboratory of Allergic Diseases > NIAID/ National Institutes of Health > 10 Center Dr., MSC-1881 > Bethesda, MD 20892-1881 > (301) 496-1306 (Voice) > (301) 480-8384 (Fax) > calman@nih.gov > RFC-822-headers: Received: from nexus.mc.duke.edu (nexus.mc.duke.edu) by mc.duke.edu (PMDF V5.0-5 #16741) id <01IHA9NDKG800038ZO@mc.duke.edu>; Thu, 03 Apr 1997 22:27:33 -0500 (EST) Received: from flowcyt.cyto.purdue.edu (flowcyt.cyto.purdue.edu [128.210.60.31]) by nexus.mc.duke.edu (8.7.5/8.7.3) with SMTP id WAA06948; Thu, 03 Apr 1997 22:24:37 -0500 (EST) Received: by flowcyt.cyto.purdue.edu (940816.SGI.8.6.9/930416.SGI.AUTO) for cyto-sendout id UAA10291; Thu, 03 Apr 1997 20:19:55 -0500 Received: from dfw-ix10.ix.netcom.com by flowcyt.cyto.purdue.edu via ESMTP (940816.SGI.8.6.9/930416.SGI.AUTO) for <cytometry@flowcyt.cyto.purdue.edu> id RAA07749; Thu, 03 Apr 1997 17:22:26 -0500 Received: (from smap@localhost) by dfw-ix10.ix.netcom.com (8.8.4/8.8.4) id QAA27870; Thu, 03 Apr 1997 16:13:06 -0600 (CST) Received: from wbr-pa2-14.ix.netcom.com(205.187.210.78) by dfw-ix10.ix.netcom.com via smap (V1.3) id sma027833; Thu Apr 3 16:12:34 1997 Date: Thu, 03 Apr 1997 17:12:30 -0500 From: Joseph Chmielowski <CHIMO@IX.NETCOM.COM> Subject: Re: anti-HLA mAb To: cyto-inbox Message-id: <199704032213.QAA27870@dfw-ix10.ix.netcom.com> MIME-version: 1.0 X-Mailer: Microsoft Internet Mail 4.70.1161 Content-type: text/plain; charset=ISO-8859-1 Content-transfer-encoding: 7bit X-MSMail-Priority: Normal X-Priority: 3
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