> From: CAWAGN@MONSANTO.COM > To: Cytometry Mailing List <cytometry@flowcyt.cyto.purdue.edu> > Subject: Re: BrdU for measuring cell proliferation > Date: Thu, 3 Apr 1997 09:06:45 -0600 > > Hello, > I need to measure the proliferation of a specific cell population > (macrophages) and think that one of the bromo-deoxy-uridine and antibody > kits would do the job. > > Does anyone have any recommendations about which manufacturer's kit does a > good job? I have experienced variable quality in purchased kits, and > would like to hear from someone who has actually used these before > investing the money. Thanks! > > Rosemarie > Dear Rosemarie, you don't need a kit for that. I use 10 mM BrdU from SIGMA in the cell media and then the monoclonal BrdU antibody from Boehringer Mannheim to do a PI-BrdU doublestaining. It works very nice and their protocol is quite simple. Ioakim Spyridopoulos, MD Dept. of Cardiovascular Research Boston, TUFTS University email: ioakim@user1.channel1.com>
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