Theolis Costa Barbosa wrote: Hi everybody, I am doing some staining for IL-2R and got surprised to see that, in spite of having many proliferating T-cells (as assessed by 7-AAD), the percents of IL-2R positive cells are low. Comments? Thank you very much for your attention. Activated, proliferating cells can shed IL-2R. An example is various forms of lymphocytic alveolitis, where the percentage of alveolar T cells expressing IL-2R is low, but soluble IL-2R may be detectable in the BAL. You don't give any more details of your system or what cells you are looking at, but maybe you could look at soluble IL2-R. Dr. D O Donnell, University College Dublin
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