Many thanks to those who had suggestions regarding my struggles with the autoclone on our 753. For anyone ever considering this, I was finally able to identify the problem, and successfully sorted into 96 well plates, although I haven't heard how the PCR went. To recap, I couldn't get anywhere with the autoclone setup because I the potentiometer meant to control the right-left positioning of the stream had no effect. (BTW, I _had_ plugged in the 3-pin connector that leads to this pot!) After some advice and a lot of meter wielding I traced this down to the number 6 pin on the sort logic card (the unique card of the 3 in the sort control module. The problem was, on 2 of the three modules I have this pin isn't connected to anything! Luckily the 3rd had a wire soldered from pin 6 to a 100k resistor! This modules still didn't work well, but after replacing 3 blown diodes everything came together. If anyone ever needs any help with this I'll be glad to share what I've learned, and thanks again to all those who offered suggestions (and sympathy). It really isn't that hard! Steve ------------------------------------------------------------- Steve G. Hilliard When a computer geek says Cell Analysis Facility "no problem" University of Georgia you KNOW you're in trouble! -------------------------------------------------------------
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