Dear flow-ers, We have been trying to do intracellular staining for p25 protein (HIV Ag) on HIV-infected cells. We have problems with cell loss after PermeaFix fixation and permeabilization and with background and/or non-specific staining (DAKO and/or Coulter p25 MoAb). Anybody out there can help us? Thanks in advance, Regards to all of you. Karolina Palucka
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