Oleg Vasilyev wrote: > >I have some flow datas of healthy patients. These datas show, that >HLA-DR negative cells have ~30 %. Other cells - HLA-DR positive are . > Most people would agree with Gary Woo that few if any T-cells in peripheral blood of normal patients express HLA-DR. This is an activation antigen which, in my experience, comes up relatively late (cells in lectin-stimulated cultures may take 48 hours or more to express it). Ther are always a few activated cells circulating in healthy people; perhaps 1% of peripheral blood T-cells might express CD25, CD71, CD98, HLA-DR, and other activation antigens. If you find 60-70% of cells from "healthy" patients to be HLA-DR positive, either your "healthy" patients are in the midst of a robust immune reaction or something went wrong with the analysis. -Howard
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