I am not feeling comfortable about the staining patterns of my Thy-1 fitc/CD34 pe staining patterns. I use thy1 from Pharmingen and CD34 from BD. The problem I see is weak Thy1 staining and low percentages. Most of what I stain are PBSC collections. If someone could let me know how much thy1 they use per 1x10-6 cells etc. If I couold recieve a fax printout of typical staining patterns, it would be greatly appreciated. thanks for any help. Jim Houston Cell and Gene Therapy St. Jude Children's Hospital Fax 901-495-2896 Ph 901-495-2926
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