Thy1/CD34 staining

From: Jim Houston (jim.houston@stjude.org)
Date: Fri Mar 28 1997 - 10:29:03 EST


I am not feeling comfortable about the staining patterns of my Thy-1 
fitc/CD34 pe staining patterns.  I use thy1 from Pharmingen and CD34 from 
BD.  The problem I see is weak Thy1 staining and low percentages.  Most 
of what I stain are PBSC collections.  If someone could let me know how 
much thy1 they use per 1x10-6 cells etc.  If I couold recieve a fax 
printout of typical staining patterns, it would be greatly appreciated.

thanks for any help.

Jim Houston
Cell and Gene Therapy
St. Jude Children's Hospital
Fax 901-495-2896
Ph 901-495-2926



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