Hello everybody I have had the same problem. If you want classy graphics you have several options and none of them are simple. There are several freeware/shareware programs that can read files created by Cell Quest. FCS Assistant ($25 shareware for the Macintosh) can read these files and convert them to ASCII. You can then plot the data in the graphing program of choice. I am not sure whether FCS assistant allows you to save gated files. I havent used it much. If you have a Windows machine, get WinMDI which lets you save histograms in ASCII. It also can save gated histograms and dot plots. Both FCS Assistant and WinMDI can be accessed from the U Mass flow cytometry software page. http://www.bio.umass.edu/mcbfacs/flowcat.html Another approach is to scan the plots of interest and then use DataThief (Available from the NIH Image site, I dont know the URL) to "steal" the x-y coordinates. This is really useful if you dont have acess to the original files and are stuck with a lousy print out. Data Thief creates a text file that can be read in any graphing program. This does not work for dot plots. Hope that helps Regards Murali Ramanathan
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