Double staining- Intracellular

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>Dear Ayala
>I understand that there is an Internet site in which FACS questions can be
>asked.
>Would you be able to post this message for me?
>Thanks.
>Danny
>
>Subject:  Double staining- Intracellular
>
>Can someone help me?
>I need to double stain for two intracellular markers:
>BrdU (nuclear) and an intermediate fillament (GFAP).
>
>I cannot use FITC and TEXAS red as conjugates, as I have no access to
>a dual laser FACS.  BUT,  I have been warned that PE and Tricolour may not
>enter the permeabilised cell due to their large size.  Any suggestions
>will be appreciated.
>Thanking you.
>
>Daniel Harari, PhD
>The Weizmann Institute
>UserID:  LCHARARI@DAPSAS1.WEIZMANN.AC.IL

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