I have been carrying out culture of CD34+ cells in the presence of IL-3, GM- & G-CSF and following myeloid differentiation using cell surface markers. I am finding that staining with CD14 is grossly under-estimating the monocytic content of these cultures as judged by morphology. One of my colleagues has made similar observations. Additionally he found that CD14+ AML cells lost expression of this marker when in culture. Has anyone had similar experiences or any good suggestions for alternative markers which more reliably indicate monocytic differentiation under these conditions? ################################################################ From: Dr Richard Darley, Ph.D. LRF Differentiation Unit Department of Haematology (A7) University of Wales College of Medicine Heath Park Cardiff, CF4 4XN U.K. tel (+44) 12 22 74 34 85 or (+44) 12 22 74 45 24 Fax (+44) 12 22 74 45 23 e-mail: darley@cf.ac.uk
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