On Wed, 19 Mar 1997, Ellen Ko wrote: > A. Fix & Perm is not the only way, but it is a good and easy way (it is > often recommended in consensus protocols)! You can buy it from caltag > in U.S. Most of our customers use this reagent! But it wont work for TdT > staining!! > This is definitely not true. We have been using Fix & Perm successfully for TdT staining using the Supertech mix of monoclonals for the last five years. Any fixation procedure will induce epitope specific alterations of the target antigens. Therefore, any fixation and permeabilization procedure can only be tested for specific clones. Also a consensus should always only be achieved on methods and quality control but never on company specific reagents. Similar reagents are by now available from most diagnostic companies. Gregor Rothe ************************************************************************* Institute for Clinical Chemistry and Laboratory Medicine University of Regensburg D-93042 Regensburg, Germany Tel. +49 (941) 944-6204 Fax +49 (941) 944-6202 Internet: Gregor.Rothe@klinik.uni-regensburg.de *************************************************************************
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