Re: Myeloperoxidase reference

From: Gregor Rothe (gregor.rothe@klinik.uni-regensburg.de)
Date: Fri Mar 21 1997 - 06:08:31 EST


On Wed, 19 Mar 1997, Ellen Ko wrote:

> A. Fix & Perm is not the only way, but it is a good and easy way (it is
> often recommended in consensus protocols)!  You can buy it from caltag
> in U.S. Most of our customers use this reagent! But it wont work for TdT
> staining!!
> 

This is definitely not true. We have been using Fix & Perm 
successfully for TdT staining using the Supertech mix of monoclonals 
for the last five years. Any fixation procedure will induce 
epitope specific alterations of the target antigens. Therefore, 
any fixation and permeabilization procedure can only be 
tested for specific clones. Also a consensus should always 
only be achieved on methods and quality control but never 
on company specific reagents. Similar reagents are by now
available from most diagnostic companies.

Gregor Rothe
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Institute for Clinical Chemistry and Laboratory Medicine
University of Regensburg
D-93042 Regensburg, Germany

Tel. +49 (941) 944-6204
Fax  +49 (941) 944-6202

Internet: Gregor.Rothe@klinik.uni-regensburg.de
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