Dear Group: I'm looking for advice on the glycophoran A (GPA) assay used to look at M and N allelic expression by flow. This is a new application for our facility. We're currently using the BR6 assay described by Langlois et al (Cytometry 11:513, 1990). However, we're having problems getting consistent light scatter profiles and with the results in general. I think part of the problem may be with the fixation. Any suggestions or comments??? Thanks. ****************************************************************************** Julie A. R. Pribyl LeBien Lab/ U of MN Cancer Center Lab phone: 612-626-4839 Flow Cytometry Phone: 612-624-7151 **************************************************************************** **
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