I have stored labeled human monocytes/macrophages under these same conditions with no detectable change in fluorescence intensity for up to a week. Kathie Woods-Cook Novartis Pharma AG ---------- From: O=internet; DDA.TYPE=RFC-822; DDA.VALUE=owner-cyto-sendout(a)flowcyt.cyto.purdue.edu To: Cytometry Mailing List; woodska1 Subject: Storage of leucocytes Date: Wednesday, 12. March 1997 01,50 <<File Attachment: BDY937.TXT>> DATE: Mar 11 14:57:55 1997 -05:00 relative to GMT IPMessageID: 9702118581.AA858121356(a)NATICK-CCMAIL.ARMY.MIL FROM: [O=internet; DDA.TYPE=RFC-822; DDA.VALUE=sgaffin(a)NATICK-CCMAIL.ARMY.MI L] TO: Cytometry Mailing List [O=internet; DDA.TYPE=RFC-822; DDA.VALUE=cytometry( a)flowcyt.cyto.purdue.edu] SUBJECT: Storage of leucocytes IMPORTANCE: normal AUTO FORWARDED: FALSE PRIORITY: ATTACHMENTS: C:\TEMP\BDY937.TXT ---------------------------------------------------------------------------- -- What is the maximum time one can store labelled leucocytes in 1% paraformaldehyde in PBS at 4oC after preparation? Thanks. Gaffin
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