My experience with selection technolgies suggests that an important factor in choosing an antigen is its degree of expression on a target cell. Improved yields result when "bright" antigens are used as the selection target. I'd go with CD14 over CD33. Tim Farley ---------- > From: Wallace Lauzon <wlauzon@uottawa.ca> > To: Cytometry Mailing List <cytometry@flowcyt.cyto.purdue.edu> > Subject: A GOOD monocyte marker? > Date: Monday, March 10, 1997 3:11 PM > > > Hi, > > I have a rather simple sounding question, particularly for the sorters > out there. I am looking to positively separate CD4, CD8, and monocytes > from Ficoll-hypaque separated blood samples. I intend to use avidin > columns and biotinylated antibodies. Obviously CD4 and CD8 will be use > for the isolation of these lymphocytes, but the best monocyted marker is > less clear. I have considered CD14, CD15, and CD33. My goal is to > obtain a pure monocyte sample, not necessarily the entire monocyte > population. The cells will be fixed immediately after they are > isolated and the subsequent manipulations are not affected by the > activation state of the cells. Does anyone have experience with these > surface markers or others that would be more appropriate for isolating a > relatively pure monocyte population? > > Thanks in advance > > Wallace Lauzon > Dept. of Microbiology and Immunology > University of Ottawa >
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