1- Are these really cells - have you SORTED them and looked under the microscope? There are CD45 DIM events (debris?) that could be sticking to your cells. 2- What sort of frequency are these events? 3- Are they positive for other markers? I don't use this clone, so I couldn't say if it's clone specific. Dennis ************************************************************************* * Dennis J. Young Voice : (619) 822-0407 * * Flow Cytometry Core Facility FAX : (619) 822-0412 * * University of California, San Diego USA e-mail: djyoung@ucsd.edu * * http://www-core.ucsd.edu/flow-core.html * ************************************************************************* ______________________________ Reply Separator _________________________________ Subject: CD45 Negative PBL Author: markrehse@CellPro.com at @UCSD Date: 3/6/97 9:42 AM Dear Flow Community, We use CD45 FITC (Immunotech clone J33) in our two color immunophenotyping panels to identify the WBC component in apheresis and buffy coat and eliminate RBC's, debris and dim staining platelets from subsequent enumeration of T and B cells, stem cells, NK cells, monocytes and granulocytes using PE-conjugated antibodies. This method is superior to traditional light scatter gating, however, there is a population of CD45- cells which are equivalent in size and granularity to T and B cells which we have not yet been able to identify in our panels. Does anyone have experience with this population? Is it a problem with this clone? Thanks in advance. MarkRehse@CellPro.com >-- Saved internet headers (useful for debugging) >Received: from flowcyt.cyto.purdue.edu by noc.ucsd.edu; id PAA09946 sendmail 8. >Received: by flowcyt.cyto.purdue.edu (940816.SGI.8.6.9/930416.SGI.AUTO) for cyt >Received: from cellpro.com by flowcyt.cyto.purdue.edu via ESMTP (940816.SGI.8.6 >Received: by cryovial.cellpro.com id <44801>; Thu, 6 Mar 1997 09:46:34 -0800 >X-Mailer: Novell GroupWise 4.1 >Date: Thu, 6 Mar 1997 09:42:09 -0800 >From: Mark Rehse <markrehse@CellPro.com >To: Cytometry Mailing L >Subject: CD45 Negative PBL >Mime-Version: 1.0 >Content-Type: text/plain >Content-Disposi >Message-Id: <97
This archive was generated by hypermail 2b29 : Wed Apr 03 2002 - 11:49:32 EST