Have any of you successfully examined DNA content in insect or other invertebrate blood cells? I have had trouble getting the cytoplasm stripped from the nuclei using lepidopteran (Manduca sexta) hemocytes. I have used fresh, unfixed cells, paraformaldehyde-fixed cells, and ethanol-fixed cells and stained with a modified Vindelov's PI. Increasing the detergent (Nonidet P-40) concentration from 0.1% to 0.2% or 0.3% didn't seem to help. I still see intact cells in the microscope. Any suggestions? Thank you very much. C. A. Hallberg Department of Entomology Purdue University West Lafayette, IN 47907
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