Dear colleagues, I perform immunophenotyping of human lymphocytes by flow cytometry. I use 1% paraformaldehyde solution (PFA; Sigma) in PBS for cell fixation. The autofluorescence of fixed cell exited at 488nm is increased 6-7 fold comparing to nonfixed control. 1. Can someone suggest me an alternative PFA source which gives minimal autofluorescence? 2. Can someone suggest me a post-fixation protocol which reduces PFA-fixed cell autofluorescence? Thank you. Dr. L. Volkov
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