A simple way to identify monocytes and macrophages which should work across species is based on lysosomal cysteine protease activity. In our experience the fluorogenic substrate (Z-Arg-Arg)2-Rhodamine 110 worked in human, rat and guinea pig samples quite well. The substrate is now available from Molecular Probes. The method which works at 488 nm has been published in Biol Chem Hoppe-Seyler 373: 547-54, 1992. Gregor Rothe ************************************************************************* Institute for Clinical Chemistry and Laboratory Medicine University of Regensburg D-93042 Regensburg, Germany Tel. +49 (941) 944-6204 Fax +49 (941) 944-6202 Internet: Gregor.Rothe@klinik.uni-regensburg.de *************************************************************************
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