(The unfortunate consequences of an inadverdent mouse click: I have to finish my thoughts in a second mail message. Pray that I don't make the same mistake again!) Using the "vertical" sort, Aaron was able to achieve greater than 90% success--measured as PCR-positive sequences from the wells. It's likely that the actual success in sorting was significantly higher than this, possibly close to 100%, and that the 10% "failures" were PCR-based failures and not sorting failures. The most difficult obstacle is the accurate positioning of the receptacle under the sorted drops. Here you may leave the cap on the plate, position it so that sorted drops end up in the right place, and then take the cap off for the sort. By the way, the vertical sorting is also useful for doing "high volume" sorts, since it builds up the least amount of charge in the receiving tube... you have much less "fly-outs" of sorted events. mr
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