A few years ago, Aaron Kantor worked out in our laboratory how to do single cell deposition into very small volumes for his single-cell, immunoglobulin sequencing project (PCR-based). He deposited individual cells into 4 ul volumes at the bottom of small eppendorf tubes; however, it should work well for Terasaki wells too. The most important feature of the setup was to offset the main stream (and main stream collector) from vertical such that the sort stream itself is vertical. The idea is to have the sorted drops falling vertically--this gives the smallest target area for the sort.
This archive was generated by hypermail 2b29 : Wed Apr 03 2002 - 11:49:31 EST