Dear all We are about to publish a paper; "Al-Rubeai, M et al. A rapid method for evaluation of cell number and viability by flow cytometry. Cytotechnology (in press)". The method is based on the ratio of cells to a known number of particles method but includes PI as a vital stain. It has been compared with traditional trypan blue stained haemocytometer counting and validated for use in animal cell biotechnology where cultures may be highly heterogeneous, containing not only "normal cells" but substantial numbers of apoptotic and necrotic cells and a large amount of debris. The method has advantages in speed, objectivity and low interobserver variation and offers the potential for near online automation in process biotechnology. Please feel free to contact me for further information. Regards DR Lloyd DR Lloyd MMedSc CBiol MIBiol Research Associate School of Chemical Engineering University of Birmingham Edgbaston Birmingham B15 2TT England t; 0121 414 5264 e; D.R.Lloyd@bham.ac.uk
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