In a manner analogous to spiking the sample with a known concentration of microspheres according to the recipe posted by Chris Groves, you could spike the sample with a known concentration of chicken erythrocytes. This concept was published by MR Loken and VL Sheckler (Transfusion 33:256-61, 1993) as part of a method for detecting low concentrations of WBC in platelet products. A commercial alternative is to use Coulter's FlowCount microspheres (concentration/mcl is already measured. They're expensive, though. > Irene J. Check, Ph.D. Director, Clinical Immunology, Flow Cytometry, and Cytogenetics Evanston Hospital 2650 Ridge Ave Evanston, IL 60201 847-570-2739 (voice) 847-570-1938 (fax)
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